Vibriosis is an important infectious venereal disease of cattle. It is a cause of infertility and abortion and is caused by the bacterium Campylobacter fetus and is spread by infected bulls when they mate susceptible cows and heifers. In this present study two buffaloes reported of repeat breeding with cervical discharges were presented to the clinics. Cervical discharges were collected and subjected for cultural isolation, microscopical examination and antibiogram. Gram negative slender rods with a characteristic flying sea gull winged appearance bacteria were observed microscopically upon Gram’s staining and dilute carbol fuschin and and the organism was isolated on MaConkey agar. Phenotypic characterization by demonstration of motility, comma shaped pink coloured rods and biochemical characterization tests like oxidase, catalase were positive and negative for indole, nitrate reduction and sugar fermentation tests suggesting firmly for confirmation of campylobacter infections.