Introduction: Normal mitral valve function depends on pillars of the trabeculae carnea represented by the papillary muscles. Knowledge of numbers of papillary muscles in anterior and posterior groups of papillary muscles is important because it is one of the components of mitral valvar complex and also for the restoration of normal physiological function of mitral valve which required resenctioned and stitching of papillary muscle, in case of injury and dysfunction of papillary muscle which lead to mitral regurgitation. Materials and Methods: Thirty formalin preserved fetal hearts at gestational age from 14 to 40 weeks were dissected through midline thoracotomy and cutting open the pericardial sac. The numbers of papillary muscles in anterior and posterior groups of papillary muscles in left ventricle were observed and recorded after an incision from the apex of heart along the left border and extending up to the left atrium. Result: The single papillary muscle in the anterior group of papillary muscles was in 21 (70%) fetuses, 2 anterior papillary muscles in 8 (26.7%) fetuses and 3 anterior papillary muscles in 1 (3.3%) fetus. The single papillary muscle in posterior group of papillary muscles was seen in 25 (83.3%) fetuses, 2 posterior papillary muscles in 4 (13.3%) fetuses, 3 posterior papillary muscles in 1 (3.3%) fetus. Conclusion: This study helps to understand the importance of papillary muscle in mitral valve complex and also to cardiothoracic surgeons for the surgery on mitral valve in case of mitral valve regurgitation.