There is a concerned growing need to increase agricultural products to meet up with growing world consumption using chemicals. Glyphosate (an herbicide) falls into the group of chemicals with perceived infertility. This study investigated the distinguishing features of glyphosate on the behaviour, body, testis and epididymis of Wistar rats. Fifteen adult male Wistar rats were randomly assigned into three groups. Rats in group A (control) received water; group B (low dose) and group C (high dose) received 400mg and 2000mg of glyphosate/kg body weight/day respectively. Experimental period lasted 60days before sacrificing. Excised testes and epididymis were fixed in Bouin’s fluid; and subjected to histological and morphometric analyses. Statistical Package for Social Scientists (version 21) was employed for analysis of collated data. The level of statistical significance was set at p < 0.05. Varying degrees of physical changes in groups B and C rats were observed. Those in group C further suffered weight loss, shedding of furs, agitation and loose stools. Glyphosate showed decreasing number of spermatozoa within the reproductive system of groups B and C rats. Dosedependent toxic effects of glyphosate on the testes and epididymides with subfertility was established probably as illustrated on the histology. Glyphosate appeared to have penetrated blood brain barrier with resultant behavioural changes among treated rats. Anti-fertility effect of glyphosate was linked to great reduction of spermatozoa density and disruption of histological characteristics of male reproductive tract. Nevertheless, the use of glyphosate should be genuinely guided or discouraged as applied in some developed countries.