Introduction: Ultrasound has become the essential tool of modern obstetric practice. Ultrasound dating along with LMP provides more accurate gestational age assessment than menstrual dating alone. Aim: To estimate gestational age in second & third trimesters using ultrasonographic imaging, by fetal biometry parameters i.e Femur Length (FL) and Humerus Length (HL). Materials and Methods: The present study was carried out in 100 normal pregnant women with singleton uncomplicated pregnancy, with the known last menstrual period (LMP). Results: FL was found to be more reliable parameter as compared to HL in both second and third trimester of pregnancy. Conclusion: HL can also be used one of the reliable parameter next to femur length in assessing gestational age.