The objective of the study is to assess the variation in splenic measurement like length, width and thickness in normal persons and categorise them according to different age group and sex using ultrasonography. This study was done on 500 individuals aged between 21-60 years. Ultrasonographic measurement of spleen was done by ESAOTE MYLAB 40 Machine. Measurement of spleen was taken by using convex 2.5-6.6 MHz probe. It was observed that the length decreased slowly with increase in age in both sexes. The width in male first it decrease then slightly increase and after that it again decreases. In female width slightly increases up to the age of 40 years, after 40 years of age it decreases slowly up to the age of 60. The thickness in male first decrease then slightly increases and after again decreases. In female first the thickness increases slowly after that it remains relatively constant, then decreases. All dimensions of spleen were greater in males that in females.