Introduction: Profunda femoris artery is the largest deep branch of femoral artery, provides the principal supply to the extensor, adductor and flexor muscles of the thigh. This large artery arises from the posterolateral side of the femoral artery about 3.5-5cm from the mid-inguinal point. The study of the femoral artery and its branching pattern has been a great interest among the anatomists and the surgeons because of its major implication in the clinical as well as radiological interventions. Material and Methods: In the present study, hundred formaldehyde embalmed cadavers without malformations were dissected and site and level of origin of the profunda femoris artery in relation to the midpoint of the inguinal ligament from the femoral artery studied, also the internal diameter of femoral artery measured by dissection method. Results: Profunda Femoris Artery originated on right side in 46% cases and 44% on left side from the posterolateral aspect. The average distance of origin of Profunda Femoris Artery from Midpoint of Inguinal Ligament on right side was observed to be 4.45 cm while on left side was observed to be 4.69cm. The mean value of Internal Diameter of Femoral Artery was found to be 7.46 mm in males and 6.33 mm in females on right side and 7.43 mm in males and 6.29 mm in females on left side. Conclusion: Most common site of origin of profunda femoris artery was observed to be the posterolateral aspect of the femoral artery. Variations seen in the form of posterior and lateral origins. The average distance of origin of profunda femoris artery was observed between 4-5cm. In the present study 3 cases of high origin of profunda femoris artery observed. The advantage of such a high origin of profunda femoris artery is that it can be used for catheterisation and further investigation of any arterial system of the body. Gender wise comparison of mean value of internal diameter of femoral artery on both sides showed statistically highly significant difference.