Introduction: Palmaris longus is a small vestigial muscle with long tendon and short belly. It is phylogenetically retrogressive. It may be absent in 15 % of individuals. Palmaris longus used as a popular tendon graft. Aim: The present study is done to assess absence of palmarislongus unilaterally or bilaterally by using simple clinical tests in both sexes on both sides. Method: We examined 200 subjects for the presence or absence of palmarislongus. Standard clinical tests were used to determine the presence or absence of palmarislongus tendon. Observations were analyzed by statistical method. Result: The study showed Palmaris longus was absent in 11 (5.5%) males and 9 (4.5%) females unilaterally. It was absent in 7 (3.5%) males and 4 (2%) females on left side. Chi square test was 0.073 and P value was 0.39 (P < 0.5). Conclusion: In present study we conclude that Palmaris longus was absent 10 % unilaterally and 6% bilaterally. Absence was more on left side than right side.