Introduction: The fibula is located lateral to the tibia in the leg. The fibula is the bone of choice for grafting and to reconstruct large defects after tumor resection, because of its length, biomechanical stability, limited donor site morbidity and predictable vascular pedicle. Objectives: The objectives of the study are: (1) to locate and describe the position, number & direction of nutrient foramina of fibula. (2) To observe any variations in location, position and number of nutrient foramina of fibula. Methods: The study sample comprised of 80 dry adult human fibulae. Present study was conducted at bone bank of Department of Anatomy, Government Medical College, Aurangabad. The data pertaining to the above parameters were noted, subjected to statistical analysis and were photographed. Results: In the present study of 80 dry adult human fibulae, noted; (1.) Absence of nutrient foramen (NF) in 2 (2.5%) fibulae. (2.) Single nutrient foramen was present in 71 (88.75%) fibulae. (3.) 5 (6.25 %) fibulae showed double nutrient foramen. (4.) 2 (2.5%) fibulae showed triple nutrient foramen. (5.) The most common location of nutrient foramen was on medial crest in 52 (58.42%) and least common on anterior border in 1 (1.25%) fibulae. (6.) Out of total 89 foramena 84 (94.67%) foramen were on the middle third of the fibula. Conclusion: The present study showed 12.7% variation in number of nutrient foramen.Accurate anatomical knowledge about the location and distribution of nutrient foramen is useful for orthopedic and plastic surgeons in planning the vascularised fibular graft at middle third of the shaft.