AbstractIntroduction: As gall bladder is often a common site of open and laparoscopic procedures, the aim of the study was to note the different congenital anomalies of Gall bladder in the adult cadavers which can provide relevant information to the surgeons and radiologists performing diagnostic and therapeutic interventions at this region. The embryological basis and clinical implications of variations are discussed. Materials and Methods: Study was carried out on 75 gall bladders of formalin fixed cadavers in department of Anatomy at K.J. Somaiya Medical College, Sion, Mumbai. The studies were carried out in the period from July 2019 to August 2021. Following parameters were noted: 1) shape 2) external morphology 3) dimensions of gall bladder which included measurement of length and breadth. Transverse diameter at the level of body of gall bladder and length from the tip of fundus to the neck of gall bladder. Observation and Discussion: In 48 specimens, gall bladder was pear shaped. Other shapes observed were Cylindrical, Hourglass, Flask shaped and Irregular. In 1 specimen, the gall bladder was partially intrahepatic. A very prominent Hartmann’s pouch was observed in 22 specimens. Length of Gall bladder ranged between 5.3 cms to 10.2 cms and transverse diameter ranged between 2.8 cms and 5 cms. Different positions of the fundus in relation to inferior border of liver were also noted. Conclusion: Anatomical variations of Gall bladder become vital during surgical settings. Congenital anomalies and anatomical variations related to the gallbladder and extra hepatic biliary tree though not common but can be of clinical importance as failure to recognize them during operative procedures may lead to inadvertent complications. It is of greatest importance to laparoscopic surgeons during cholecystectomy to prevent alarming consequences as failure to recognise them can lead to iatrogenic injuries and increase morbidity and mortality. Every surgeon should assess for these anomalies before any operative procedures