Variations are very commonly found in different vessels of the body. Gonadal vessels also show numerous variations which are important in surgeries of posterior abdominal wall. Also, these vessels are closely related to renal vessels, so, variable origins of these vessels are of utmost importance in renal surgeries. Present study was undertaken to observe the variations in the gonadal vessels. Materials and Methods: 30 cadavers (26 males and 4 females) embalmed in 10% formalin were procured from the Anatomy Department of, D Y Patil Medical College, Pune. So, sixty gonadal vessels were dissected for this study. The variable levels of origin of gonadal arteries from abdominal aorta as well as variable origins of the same were noted down. Also, the variable draining pattern of gonadal veins were noted. Photographs of all the variations were taken. Results: Numerous variations in origin, level and source of origin of testicular arteries and ovarian arteries were found. Common variation found was their origin from accessory renal arteries. Gonadal veins also showed, variable draining patterns with sometimes duplication of veins. Conclusion: Variant origin and draining pattern of gonadal vessels are important in the surgeries of posterior abdominal wall, renal surgeries like renal transplant, surgical excision of tumors of posterior abdominal wall, surgeries on abdominal aorta etc. Also, these variations are important for radiologists and also, for surgeries of varicocoele and testicular tumors.