The present study highlights the need to “A study to assess the knowledge regarding selected organs donation among healthcare professionals and non-healthcare professionals in selected areas at Ahmadabad.” 60 (30- healthcare professionals and 30 non healthcare professionals) samples were selected by convenience sampling technique. Data was collected using self structured questionnaire assessing the knowledge. The data was analysed and interpreted by using descriptive and inferential statistics. Majority (90%) of the healthcare professionals had average knowledge, 10% of them had good knowledge 100% of the healthcare professionals.