An experimental study was conducted to evaluate the Effectiveness of Planned Teaching Programme on knowledge regarding Prevention of Nosocomial Infection among Staff Nurses working in selected hospital at Gwalior. The investigator has selected the   experimental evaluative research approach. The study was conducted among Staff nurse of district hospital Morar Gwalior, MP. The random sampling technique was apprpopriate to the study and sample size was 40. In present study pre test –47.5% Staff Nurses have inadequate knowledge, 37.5% Staff nurses have moderate knowledge, 15% staff Nurses have adequate knowledge. In post test 37.5% Staff nurses have moderate knowledge, 70% staff Nurses have adequate knowledge and it also shows that the staff nurses having improvement in their knowledge. The analysis revealed significant association between the pre test knowledge score with demographic variables like age, qualification clinical experience during staff nurse period at the level of p<0.05.