Background: HER-2/neu is a gene localized on chromosome 17q21 which encodes a growth factor receptor like molecule with tyrosine kinase activity and is known to encode a transmembrane glycoprotein. Aim: To study the expression of HER2/neu in benign and malignant lesions of cervix. Material and Methods: The present study was conducted on 100 cases of resected cervical tissue specimen suspected to have neoplastic lesions and hysterectomy specimen already diagnosed as carcinoma on cytology for a period of 12 months (September 2018 to August 2019). Results: Majority of benign cases were negative for HER-2/neu except few cases and positivity was higher in malignant than premalignant. Positivity was also higher in adenocarcinoma as compared to squamous cell carcinoma. Conclusion: HER-2/neu serves as a biological marker to identify high risk subgroups and can be useful as target for new treatment modalities.