Introduction: In general, chronic cholecystitis associated with cholelithiasis features dense mural muscular hypertrophy and fibrosis, and usually only minimal chronic inflammation. Rokitansky–Aschoff sinuses are frequently identified; these represent diverticuli of gallbladder mucosa into the muscular layer, which are associated with focal mild muscular hypertrophy. Methodology: The study mainly included all cholecystectomy specimens received at the Department of Pathology. The patient’s name, age, sex, parity, diet and clinical features were noted. Some of the cases had been diagnosed clinically and all cases had been subjected to ultrasound examination preoperatively. Results: Microscopically, 104 (57.1%) cases had normal mucosal lining, 38 (20.9%) had atrophied lining, 24 (13.2%) had ulcerated mucosa, 12 (6.6%) had hyperplastic lining and two each had necrosed mucosal lining (1.1%) and pyloric gland metaplasia(1.1%). Conclusion: Wall was infiltrated with lymphocytes, plasma cells, eosinophils and macrophages and showed fibrosis and congested blood vessel. Mild degree of inflammation was seen in 97 (53.3%), 59 (32.4%) had moderate and 26 (14.3%) had severe inflammation. Rokitansky Ascoff Sinuses was seen in all cases. Key words: Chronic Cholecystitis; Rokitansky Ascoff Sinuses; Cholelithiasis.