Introduction: Barr body has been recognized as the cytological manifestation of the inactive X chromosome in interphase nuclei in a highly condensed state. Aims and Objectives: To assess the efficacy of sex determination between pap stained buccal mucosal scrapes and leishman stained drumstick appearance in peripheral smear. Material and Methods: Buccal mucosal smears and peripheral smears are collected from 100 individuals who visit K. R. hospital and Cheluvamba hospital of age ranging from 20-30 years. The buccal mucosal scrapes were stained with papanicolaou stain and peripheral smear of the same individual were stained with leishman stain. Results: In females and males drumstick appearance was more compared to barr body. Conclusion: Sex determination using buccal scrapes and peripheral smear stained with papanicolaou stain and leishman stain proved to be simple, accurate and cost effective method in our study.

Keywords: Barr Body; Drumstick; Papanicolaou Stain; Leishman Stain.