Advertisement!
Author Information Pack
Editorial Board
Submit article
Special Issue
Editor's selection process
Join as Reviewer/Editor
List of Reviewer
Indexing Information
Most popular articles
Purchase Single Articles
Archive
Free Online Access
Current Issue
Recommend this journal to your library
Advertiser
Accepted Articles
Search Articles
Email Alerts
FAQ
Contact Us
Journal of Microbiology and Related Research

Volume  3, Issue 2, Jul-Dec 2017, Pages 107-112
 

Original Article

To Characterize & Determine the Virulence Factors of Staphylococcus aureus in a Tertiary Care Hospital

Dalip K. Kakru*, Mohd Suhail Lone**, Bakhshi Fariyal***, Junaid Ahmad****

*Professor & Head ****Senior Resident, Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Srinagar, Jammu and Kashmir 190011, India. **Assistant Professor, Department of Medical Microbiology, JNU Hospital & Medical College (JNUIMSRC), Jaipur, Rajasthan 302017, India. ***Clinical Biochemistry, Kashmir University, Srinagar, Jammu and Kashmir 190006, India.

Choose an option to locate / access this Article:
90 days Access
Check if you have access through your login credentials.        PDF      |
|

Open Access: View PDF

DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3217.10

Abstract

Context: Staphylococcus is a gram-positive, non-motile, nonsporing bacteria that include different opportunistic pathogenic species, responsible for human and animal infections. Aims: To isolate and identify Staphylococcus aureus from different human clinical samples and to determine various virulence factors of these isolates. Settings and Design: Prospective study conducted in Dept of Microbiology, SKIMS J&K. Methods and Material: Various clinical samples eg pus, blood, sputum etc received in the Microbiology laboratory were processed for isolation of Gram positive cocci. Identification of Staphylococcus aureus was done by standard microbiological methods and various virulence factors were determined. Statistical Analysis Used: Descriptive statistics (frequency and percentage) was used. Results: A total of 217 strains of Staphylococcus aureus were isolated from 1100 clinical specimens. All the isolates had ability to produce free and bound coagulase enzyme. Out of 217 strains, 42 (19.35%)strains showed only  hemolysin production, 84 (38.70%) strains showed only  hemolysin production and rest 91(41.93%) strains showed both  and hemolysin production. Among all the strains, 203 (93.54%) were positive for producing biofilm and rest of 13 were negative for producing biofilm. Out of 217 strains of S.aureus only 49 (22.58%) strains were able to produce the staphyloxanthin pigment and rest 168 (77.41%) were found to be negative. All strains of S.aureus were sensitive to Linezolid, Teicoplanin and vancomycin. On the other hand strains showed high resistance towards Cefoxitin (65.89%). Conclusions: Prevalence of methicillin rsistance was high in IPD setting (65.89%) and production of various virulence factors like coagulase, hemolysin production, biofilm production and staphyloxanthin pigment production was more common in MRSA than in MSSA thus suggesting that infection control policies should be adhered positively.

Keywords: Staphylococcus Aureus; Prevelance; Virulence Factors. 


Corresponding Author : Dalip K Kakru, Professor & Head, Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Srinagar, Jammu and Kashmir 190011, India.