Vannila planifolia Andrews is one of the most important flavouring herbaceous perennial crop. In vanilla in vitro propagation study was conducted to assess the in vitro response of various explants in different growth regulators to identify the ideal and suitable medium for primary callus induction and to found out the response of in vitro regeneration of plantlets from callus tissue. Three different explants viz., Nodal segment, shoot tip and aerial tip from healthy plants were undertaken for this study. Explants were inoculated in MS medium (Murashige and Skoog 1962) supplemented with BAP, IAA, D-biotin, Calcium pentothenate for primary callus initiation. The growth of multiple shoots was more vigorous on MS medium with 0.2 mg/L of KN and 0.1 mg/L of NAA. MS medium with 1.5 mg/L of IBA and 1.0 mg/L of BA gave good results for rooting. The explant nodal segment gave best response both early shoot induction and higher shooting percentage and also earlier rooting induction with rooting percentage.

Keywords: Micro propagation; Nodal segment; Shooting; Rooting.