Introduction: Breastfeeding is the normal way of providing young infants with the nutrients they need for healthy growth and development. Exclusive breastfeeding is recommended up to 6 months of age, with continued breastfeeding along with appropriate complementary foods up to two years of age or beyond. Objective: The objective of the study was to assess the knowledge on breast feeding among antenatal mothers and to associate the knowledge on breast feeding among antenatal mothers with their demographic variables. Methodology: Quantitative research and descriptive design was adopted for the study. A total of 50 samples were selected using non probability purposive sampling technique at Radhapuram block Primary health centre, Villupuram district. The tool used for the study comprises of 2 sections, section A: Demographic data which consist the items for obtaining information about the selected Background factors such as age, Type of gravida, educational status, Religion, Number of children’s, monthly income, place of residence, types of Family, occupation, source of health information and availability of health facilities. section B: Structured knowledge questionnaire developed by the investigator which consist of 30 items pertaining to the knowledge regarding breast feeding. Results: The study findings revealed that mother’s knowledge regarding breast feeding is inadequate. Only 4% (2) of antenatal mothers had adequate knowledge about breast feeding , 32% (16) had moderate knowledge and 64% (32) had inadequate knowledge regarding breast feeding. Conclusion: It is essential, that accurate information and education should be given to mothers and caregivers about appropriate timing of initiating breast feeding practices which helps to prevent malnutrition and improve the health status of the children.