Introduction: Newborn care is the care which is given immediately after the birth. Newborn care includes warmth, cord care, eye care, breasting feeding and immunization. Objective: To assess the knowledge on newborn care among primigravida mothers. Materials and Methods: Quantitative research approach and descriptive research design was adopted. A total of 50 samples were selected by using non probability purposive sampling technique in SRM Rural Health Center at Mamandur village. Structured knowledge questionnaire was used to assess the level of knowledge on newborn care. The collected data was analyzed by using descriptive and inferential statistics. Results: The results of the study revealed that 36(72%) mother had moderately adequate knowledge, 14 (28%) mother had adequate knowledge and none of them had inadequate knowledge on newborn care. and there is a significant association on the level of knowledge among primigravida mothers with their demographic variables of family income and type of family. Conclusion: Most of the primigravida mothers had moderately adequate knowledge, Hence it is necessary to enhance their knowledge through an educational approach.